Determination of the lipid composition of the GPI anchor.
In eukaryotic cells, a subset of cell surface proteins is attached by the glycolipid glycosylphosphatidylinositol (GPI) to the external leaflet of the plasma membrane where they play important roles as enzymes, receptors, or adhesion molecules.Here we present a protocol for purification and mass spectrometry analysis of the lipid moiety of individu